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KLE細胞，人子宮內(nèi)膜腺癌細胞

簡要描述：KLE細胞，人子宮內(nèi)膜腺癌細胞
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  • 產(chǎn)品型號：CRL-1622
  • 廠商性質(zhì)：生產(chǎn)廠家
  • 更新時間：2024-11-21
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KLE細胞，人子宮內(nèi)膜腺癌細胞

ATCC® Number:CRL-1622™    Price:$338.00
Designations:KLE

Depositors:GR Richardson

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:


Source:Organ: uterus
Tissue: endometrium
Disease: adenocarcinoma

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
KLE細胞，人子宮內(nèi)膜腺癌細胞

Tumorigenic:Yes

Antigen Expression:blood type O; Rh+

DNA Profile (STR):Amelogenin: X

CSF1PO: 13,14

D13S317: 12

D16S539: 11,12

D5S818: 9,12

D7S820: 11,12

THO1: 6,7

TPOX: 8,11

vWA: 16



Age:64 years adult

Gender:female

Ethnicity:Caucasian

Comments:Electron microscopy of tumors formed in nude mice shows microvilli and junctional complexes, and nucleolar channel systems are present that are similar to those seen in normal endometrium under progestational stimulation. The tumors do not form glands.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C

Subculturing:Protocol:                    

  1. Remove and discard cult   ure medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week

Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105
















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