丝袜美腿美女被狂躁动态图片,乖乖 张大点就不疼了,雷电将军乳液狂飙vx免费进入,荆棘花园 阿色

咨詢熱線

15000266580

當前位置：首頁 >產(chǎn)品中心>細胞庫>人腫瘤細胞、癌細胞>CCL-138Detroit 562細胞， 人咽頭癌胸水轉(zhuǎn)移細胞

Detroit 562細胞， 人咽頭癌胸水轉(zhuǎn)移細胞

簡要描述：Detroit 562細胞， 人咽頭癌胸水轉(zhuǎn)移細胞
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號：CCL-138
  • 廠商性質(zhì)：生產(chǎn)廠家
  • 更新時間：2024-11-16
  • 訪  問  量：1427

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細介紹

Detroit 562細胞， 人咽頭癌胸水轉(zhuǎn)移細胞

OrganismHomo sapiens, human
Tissuepharynx: derived from metastatic site: pleural effusion
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level1
Diseasepharyngeal carcinoma
Age*****
Genderfemale
EthnicityCaucasian
Storage Conditionsliquid nitrogen vapor phase

 Karyotype   Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines   Clinical Data  

Detroit 562細胞， 人咽頭癌胸水轉(zhuǎn)移細胞

Caucasian

female

 Genes Expressed  

keratin

 Virus Susceptibility   Human poliovirus 1
Vesicular stomatitis virus              
 Comments  

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing





Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage Temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C              CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細胞























產(chǎn)品咨詢

留言框

  • 產(chǎn)品：

  • 您的單位：

  • 您的姓名：

  • 聯(lián)系電話：

  • 常用郵箱：

  • 省份：

  • 詳細地址：

  • 補充說明：

  • 驗證碼：

    請輸入計算結(jié)果（填寫阿拉伯數(shù)字），如：三加四=7

聯(lián)系方式

郵箱：xiangfbio@163.com

地址：上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

（周一至周日9:00- 19:00）

在線咨詢

  • 掃一掃 微信咨詢

Copyright©2024 上海復祥生物科技有限公司 All Right Reserved    備案號：滬ICP備10013034號-2     sitemap.xml
技術(shù)支持：化工儀器網(wǎng)    管理登陸